LOCUS       sequence-96609-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc Separase C2029A in the genome and 
            Tet-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Holland AJ, Taylor SS
  TITLE     Cyclin-B1-mediated inhibition of excess separase is required for 
            timely chromosome disjunction.
  JOURNAL   J Cell Sci. 2006 Aug 15;119(Pt 16):3325-36. Epub 2006 Jul 25.
  PUBMED    16868023
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-08-15"; volume: "119(Pt 16)"; pages: "3325-36. 
            Epub 2006 Jul 25"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase C2029A
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     38..62
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             323..352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
       61 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      121 gcctccggac tctagcgttt aaacttaagc ttggtacccc ggggaattcg gcagatcccg
      181 aggtccgaca gcgcccggcc cagatcccca cgcctgccag gagcaagccg agagccagcc
      241 ggccggcgca ctccgactcc gagcagtctc tgtccttcga cccgagcccc gcgccctttc
      301 cgggacccct gccccgcgga tggagcagaa gctcatctca gaagaagacc tcgagaggag
      361 cttcaaaaga gtcaactttg ggactctgct aagcagccag aaggaggctg aagagttgct
      421 gcccgacttg aaggagttcc tgtccaaccc tccagctggt tttcccagca gccgatctga
      481 tgctgagagg agacaagctt gtgatgccat cctgagggct tgcaaccagc agctgactgc
      541 taagctagct tgccctaggc atctggggag cctgctggag ctggcagagc tggcctgtga
      601 tggctactta gtgtctaccc cacagcgtcc tcccctctac ctggaacgaa ttctctttgt
      661 cttactgcgg aatgctgctg cacaaggaag cccagaggcc acactccgcc ttgctcagcc
      721 cctccatgcc tgcttggtgc agtgctctcg cgaggctgct
//
