LOCUS       sequence-96637-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Splitposon in KanR ColE1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_1
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..107
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(113..119,120..135,136..141))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        660..737
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 taggcgtatc acgaggcaga atttcagatc tcatatacct cactcaaaat gcgaaagcgt
       61 ttcacgataa atgcgaaaac ggatcctaat tgtgagcgct cacaattcca cacattatac
      121 gagccgatga ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt
      181 cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg
      241 atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg
      301 gtgcaccgtg cagtcgataa gcccggatct cgaccgatgc ccttgagagc cttcaaccca
      361 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
      421 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
      481 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
      541 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
      601 attatcgccg gcatggcggc caattcgcgc gcgaaggcga agcggcatgc atttacgttg
      661 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
      721 aattcagggt ggtgaatgtg aa
//
