LOCUS       sequence-96664-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            trimethoprim resistance gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2ST
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tacgccccgc cctgcttaac ccttttgcca gatttggtaa ctataattta
      181 tgttagaggc gaagtcttgg gtaaaaactg gcctaaaatt gctggggatt tcaggaaagt
      241 aaacatcacc ttccggctcg atgtctattg tagatatatg tagtgtatct acttgatcga
      301 tcaggctttt gtatatctcc ccaccacctg aaacaatgac atgatccgtt attttcttta
      361 ggttggttaa agcatcttta attgatggaa agatcaatac gtcctcattg tcagatgtaa
      421 aacttgaacg tgttacgacc gcatactttc ggttgggtaa tgctcccatt gattcaaaag
      481 tcttgcgtcc aaccaacagc cattggttat aggtaatagc tttaaacagg agctgttcac
      541 ctttggcact ccatggaata tcagggccat tcccgataac tccattcttc gatatagcta
      601 ccattagtga tagtttcaca attcttcctc agaggttaac attttattag taagcatgct
      661 cgttttgtca cccgctgatg cttaccgtta attaatataa tcacttgtat taaatagact
      721 ttctttaaaa atacaagaca ctctgttatt acaaatcgtg catgccgtct atccgattac
      781 cctgttatcc ctagctgaca ctgaggatct acttacatac tgctcgcgat aactgactct
      841 gccactcaag accaaaaaaa accccgccct gtcaggggcg gggttttttt tttgttaggt
      901 cagtgacctc t
//
