LOCUS       sequence-96704-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg agctctaata
       61 cgactcacta tagatgggcg gcgcatgaga gaagcccaga ccaattacct acccaaaatg
      121 gagaaagttc acgttgacat cgaggaagac agcccattcc tcagagcttt gcagcggagc
      181 ttcccgcagt ttgaggtaga agccaagcag gtcactgata atgaccatgc taatgccaga
      241 gcgttttcgc atctggcttc aaaactgatc gaaacggagg tggacccatc cgacacgatc
      301 cttgacattg gaagtgcgcc cgcccgcaga atgtattcta agcacaagta tcattgtatc
      361 tgtccgatga gatgtgcgga agatccggac agattgtata agtatgcaac taagctgaag
      421 aaaaactgta aggaaataac tgataaggaa ttggacaaga aaatgaagga gctggccgcc
      481 gtcatgagcg accctgacct ggaaactgag actatgtgcc tccacgacga cgagtcgtgt
      541 cgctacgaag ggcaagtcgc tgtttaccag gatgtatacg cggttgacgg accgacaagt
      601 ctctatcacc aagccaataa gggagttaga gtcgcctact ggataggctt tgacaccacc
      661 ccttttatgt ttaagaactt ggctggagca tatccatcat actctaccaa ctgggccgac
      721 gaaaccgtgt taacggctcg taacataggc ctatgcagct ctgacgttat ggagcggtca
      781 cgtagaggga tgtccattct ta
//
