LOCUS       sequence-96705-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(444..503)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 accccgaccc cgctcgccat gctattgccg ccgggggcca gcagcccgcc gggcagcgtg
       61 tacttatcct tcttcatgag cgtcttggtt ttccgccggg gccggtattt ataatccggg
      121 tgctccttca tgtgcagcgc tcgcagccgc ttagcctcgt cgatgaacgg ccgcttctcc
      181 gtctccgaca aaagtttcca ctcggcgccc aggcgcttgc tgatctccga gttgtgcatc
      241 ttggggttct cctgggccat cttgcgccgc tgcccgcggg accacaccat gaaggcattc
      301 atgggccgct tgacgcggtc cgggctgttt ttctggttgc cgccggccgc cgccgcggtg
      361 gagttgccgc cgccgccccc cgaagtttgc tgcgggcccg gcggcttcag ctccgtctcc
      421 atcatgttgt acatgggcgc gccaggaccg gggttacttt caacatcgcc agcgagtttc
      481 aacaaagcgt agttagtaca ttgtctagaa aaatgcctct tcatgtgtaa ggcgaggtgg
      541 tccgacctgg aaaatgctcg gtcgcatttt tggcactgga acgggcggtg ccccgtgtgt
      601 ttacggtagt gcctggtcag
//
