LOCUS       sequence-96763-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of SV40 large T antigen and mCherry red 
            fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pRRLsin-SV40 T antigen-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..66
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..66
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(364..383)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     633..651
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aatctttcac aaattttgta atccagaggt tgattgtcga cgcggccgct ctagaactag
       61 tggatccccc gggctgcagg aattcttact tgtacagctc gtccatgccg ccggtggagt
      121 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
      181 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
      241 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
      301 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
      361 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
      421 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
      481 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
      541 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
      601 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
      661 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
      721 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
      781 cgcccttgc
//
