LOCUS       sequence-96776-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: Empty sgRNA target (CspCI placeholder) (see 
            comments)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ374
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..783
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgccctacaa caactaagaa
       61 aatggctatc atgcggaagc tggtgagaag aacagcatcg ggacaaggga aggaagaaca
      121 aagacaaaga aaacaaaaga aagcaattga aaacaaaaca aaacaatttt cattccttct
      181 cttatcattc cttttctttt cttttctctc attcaacgca ctccatcgta tccgtattcc
      241 tcttattttt tctctttctc tatatccatt tctttctctc taggtgtgtc ctctctctct
      301 cttcaatttc tctactccgc attccaacgc atccttcccc caacctccca tttcctcctt
      361 acggcccgat agcgatcgtc tttccctcgc tatcactcgc taccggcccc tcctctgcac
      421 cgtaacctcc tacgtattta ccatatcata aagttttttc cgacgcttat cgctgacccc
      481 ctgtcgccct cctattggct tccggattat cttcttgtcc ataaggtgat ccatgcttcc
      541 tgaagattcc cgaaatgtgt ccactttggc ggggaatcat tccatccact tctttctctc
      601 tcgctttcct cattcggcgc tccccttccg cgtctcattg gtcttccgct ccgtttttgc
      661 tttgccgatg ttacttgggg agaggtgcga taatcctttc gcaaaaactc ggtttgacgc
      721 ctcccatggt ataaatagtg ggtggtggac aggtgccttc gcttttcttt aagcaagaga
      781 attgctgcag gtcgactcta gagatggaca agaagtactc catt
//
