LOCUS       sequence-96808-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT with AhyI insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-AhyI
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..91
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(106..111,112..129,130..136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgtagcg
       61 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      121 tccggctcgt atgttgtgtg gaattgtgag cgggataaca atttcacaca ggatctcgac
      181 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
      241 ttaaccgtcg ccgggatgct ggggccgtca ttgctaacgc cgcgacgtgc gactgcgcat
      301 atggaattca tgttggtatt caaaggtaag ttgaaagagc acccacgttg ggaagttgaa
      361 aacgaattat acaggtttag aaaccgtgta ttctcagata gattgggatg ggatgttgag
      421 agtcacaggg gtctagagca ggactctttc gacaccccag atacgcattg ggtgttaatc
      481 gaagacgagg aagggttgtg tggttgcatc cgtcttctta gttgtgcgaa agattacatg
      541 ctgccaagta tattccccac cgctcttgca ggtgaggcgc ctcccagaag caacgatgtc
      601 tgggagctga ccaggctagc catagatgcg gagagggctc cgagactagg caacggtatc
      661 tcagaactaa catgcatcat ctttagggaa gtctacgcat tcgcaaaagc gcagggcatc
      721 agggaactgg ttgcggtggt ttcactgccg gttgaaagga tttttcgtag gttgggccta
      781 cctatcgagc gtcttggtca ccgtcaagca gtggattta
//
