LOCUS       sequence-96837-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against human CTR1 from puromycin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pSUPER-retro-puro-tetO-CTR1-shRNA
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(97..113)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(98..114)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(132..548)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(148..164)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(180..202)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ttataagttc cctatcagtg atagagatcc ccaaagccca gctttctctt tggttcaaga
       61 gaccaaagag aaagctgggc ttttttttaa gcttatcgat accgtcgacc tcgagagatc
      121 gatctaattc cggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg
      181 gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc
      241 ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc
      301 acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta
      361 acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt
      421 ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg
      481 tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca
      541 gtctggccct aattttcaga caaatacaga aacacagtca gacagagaca acacagaacg
      601 atgctgcagc gctgcagcag acaagacgcg cggcttcggt tccaaaccga aagcaaaaat
      661 tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatcctgac
      721 ggggtcggat tccacatcga ctcccttcct caggtcgggc cacaaaaac
//
