LOCUS       sequence-96850-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of human JUND.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pLKO.1 shJUND puro
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    301..418
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        467..977
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     896..914
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccgggcg cctggaagag aaagtgaact cgagttcact
      241 ttctcttcca ggcgcttttt aattctcgac ctcgagacaa atggcagtat tcatccacaa
      301 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      361 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      421 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
      481 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      541 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      601 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      661 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      721 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
      781 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cggaaggggc
      841 ggtgcgggaa ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
      901 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
      961 ccgacctctc tcccagggga ccaccggagc ttccatgacc cgagtacagc ccacggggcg
     1021 cctcgccccc gcga
//
