LOCUS       sequence-96886-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-Tre for expression of traptavidin (high stability mutant of 
            streptavidin) bearing a glutamic acid tag for ion-exchange 
            chromatography.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Traptavidin-E6
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             468..485
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(538..556)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      552..599
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat ggctgaagct ggtatcaccg
       61 gcacctggta caaccagctg ggatccacct tcatcgttac cgctggtgct gacggtgctc
      121 tgaccggtac ctacgaatcc gctgttggta acgctgaagg cgattacgtt ctgaccggtc
      181 gttacgactc cgctccggct accgacggtt ccggaaccgc tctgggttgg accgttgctt
      241 ggaaaaacaa ctaccgtaac gctcactccg ctaccacctg gtctggccag tacgttggtg
      301 gtgctgaagc tcgtatcaac acccagtggt tgttgacctc cggcaccacc gaagctaacg
      361 cgtggaaatc caccctggtt ggtcacgaca ccttcaccaa agttaaaccg tccgctgctt
      421 ccgaagagga agaggaagag taataaaagc ttgcggccgc actcgagcac caccaccacc
      481 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      541 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      601 tgaaaggagg aactatatcc ggattggcga atg
//
