LOCUS       sequence-96896-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_smFP V5
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             152..193
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             203..244
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             254..295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
       61 atcattttgg caaagaattc ggtaccgcgg gcccggcgcg ccagctggat atctccggac
      121 ccgggctagc gcaattgcgg ccgccaccat gggaaaacct ataccgaacc ccctccttgg
      181 acttgatagt actcagcagc agggtaagcc cattccaaac ccacttctcg gtctggatag
      241 cacacagcaa caaggtaagc ctatccctaa ccctctgttg ggcctggact ccacgggcca
      301 aggtgtgagt aagggcgaag aactgtttac cggcgtggtc ccaattctgg ttgagctcga
      361 cggtgacgtt aacggacaca agtttagtgt gcgcggagaa ggagagggag atgccaccaa
      421 cggaaaattg acgctgaagt ttatatgcac cacgggaaag ctgccggtgc cgtggccgac
      481 cttggttaca accctgggtg gcggagtcca gtgtttcagc cgttacccag accacatgaa
      541 gcagcatgac ttctttaaga gtgctatgcc ggagggatac gtgcaggaac ggacgatcag
      601 tttcaaagat gacggtactt acaagacgcg agccgaggtg aagttcgagg gagacacctt
      661 ggtgaacagg atagaactta agggaattga tttcaaggaa gacggtaata ttctgggtca
      721 caagctggaa tacaacttta acagccataa cgtctacatt actg
//
