LOCUS       sequence-96915-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA to mouse Lmo4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Lasek AW, Kapfhamer D, Kharazia V, Gesch J, Giorgetti F, Heberlein U
  TITLE     Lmo4 in the nucleus accumbens regulates cocaine sensitivity.
  JOURNAL   Genes Brain Behav. 2010 Oct;9(7):817-24. doi: 
            10.1111/j.1601-183X.2010.00620.x. Epub 2010 Aug 12.
  PUBMED    20618444
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1601-183X.2010.00620.x"; journalName: "Genes Brain 
            Behav"; date: "2010-10"; volume: "9"; issue: "7"; pages: "817-24"
COMMENT     Sequence Label: pLL3.7 Lmo4.3 shRNA
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     148..168
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     284..300
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     285..301
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(343..376)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tattgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
       61 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataagagata
      121 caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag
      181 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttg tgtttcacat
      241 gttctacctt caagagaggt agaacatgtg aaacactttt ttctcgaggt cgacggtatc
      301 gataagctcg cttcacgaga ttccagccgg tcgagggacc taataacttc gtatagcata
      361 cattatacga agttatatta agggttccaa gcttaagcgg ccgcgtggat aaccgtatta
      421 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
      481 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      541 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      601 cattgacgtc catgggtgga gtatttacgg taaactgccc acttggca
//
