LOCUS       sequence-96947-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA for cleavage at unc-58(e665) locus in C elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ
  TITLE     Efficient Marker-Free Recovery of Custom Genetic Modifications with 
            CRISPR/Cas9 in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Aug 26. pii: genetics.114.169730.
  PUBMED    25161212
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Aug 26. pii: genetics.114.169730."
COMMENT     Sequence Label: pJA50
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..49
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     33..49
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        224..573
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     misc_RNA        594..669
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(812..828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(812..828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttgattttc taccgaagaa aggcccaccc gtgtaaaacg acggccagtt tatctagtca
       61 gcttgattct agctgatcgt ggaccggaag gtgagccagt gagttgattg cagtccagtt
      121 acgctggagt ctgaggctcg tcctgaatga tatgcgaccg ccggagggtt gcgtttgaga
      181 cgggcgacag atccagtcgc gctgctctcg tcgatccaag cttcaaaaaa aactagcaat
      241 aaaggaataa aaaactgtac accttaaagg cgcacactct gttttgcaaa ttttattttt
      301 agttgtgaat tttctgctga gacttgaaaa tagcaacttt agtactacta taatttgtca
      361 accttttcaa aaaaagcatg caatttttga gaaactctta taaaagctat tattaaaaaa
      421 acaccttttt tccaaaatta ttccacaaaa aatatgttat gaaatgccta caccctctca
      481 cacacactct ttatactact ctgtcaaact cacgagatgt ctgccgcctc ttgtgttgcc
      541 cctatataaa cacctcctat tgcgagatgt cttgtccacg cacatggtca ctagttttag
      601 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      661 agtcggtgct ttttgtgaaa tttgctagcg gtgcgagcgg atcgagcagt gtcgatcact
      721 actggaccgc gagctgtgct gcgacccgtg atcttacggc attatacgta tgatcggtcc
      781 acgatcagct agattatcta gtcagcttga tgtcatagct gtttcctgag gctcaatact
      841 gaccatttaa atcatacctg acctccatag cag
//
