LOCUS       sequence-97021-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Nickerson A, Huang T, Lin LJ, Nan X
  TITLE     Photoactivated localization microscopy with bimolecular fluorescence
            complementation (BiFC-PALM) for nanoscale imaging of protein-protein
            interactions in cells.
  JOURNAL   PLoS One. 2014 Jun 25;9(6):e100589. doi: 
            10.1371/journal.pone.0100589. eCollection 2014.
  PUBMED    24963703
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-25"; volume: "9(6):e100589. doi"; pages: " 
            10.1371/journal.pone.0100589. eCollection 2014"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1_1-159-MCS
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(302..320)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     570..589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atcacatcaa atatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccgc agccgccccc
      121 ttcaccaagc ttctcgagct gcaggagctc ggtaccatgg tgagcaaggg cgaggaggat
      181 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc atatggaggg ctccgtgaac
      241 ggccacgtgt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      301 gccaagctga aggtgaccaa gggtggcccc ctgcccttca cctgggacat cctgtcccct
      361 cagttcatgt acggctccaa cgcctacgtg aagcaccccg ccgacatccc cgactacttc
      421 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaagttcga ggacggcggc
      481 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      541 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      601 gaggccctct ccgagcggat gtaccccgag gactaaaagg gtgggcgcgc cgacccagct
      661 tttcttgtac aaagttggca ttataagaaa agcattgctt atcaatttgt tgcaa
//
