LOCUS       sequence-97076-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVET-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(199..218)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
       61 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
      121 ataccatggc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
      181 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
      241 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
      301 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
      361 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
      421 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
      481 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
      541 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
      601 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
      661 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggatt
//
