LOCUS       sequence-97107-e        8599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enhance solubility and correct disulfide bond-formation of fused 
            protein expressed in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong 
            B, Kim SW, Choe H
  TITLE     Expression and purification of biologically active human FGF2 
            containing the b'a' domains of human PDI in Escherichia coli.
  JOURNAL   Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 
            10.1007/s12010-013-0140-3. Epub 2013 Mar 8.
  PUBMED    23471584
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12010-013-0140-3"; journalName: "Appl Biochem Biotechnol";
            date: "2013-05"; volume: "170"; issue: "1"; pages: "67-80"
COMMENT     Sequence Label: pDEST-hPDI
FEATURES             Location/Qualifiers
     source          1..8599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..631
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     510..529
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(672..796)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             810..827
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(880..898)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      894..941
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      978..1433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1065..1084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1275..1296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1460..1564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1565..1633,1634..2425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1783..1802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2596..3184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3085..3104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3338..3355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3370..3512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3438..3457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3598..3620
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3614..3805)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4614..5696)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5658..5677
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5697..5774)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5980..5999
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6089..6108
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6089..6107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6108..6132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6147..6169
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6182..6205
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    7691..7815
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7840..7845,7846..7863,7864..7870)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7924..8583
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7990..8009)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
       61 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
      121 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
      181 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
      241 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
      301 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
      361 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
      421 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
      481 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
      541 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
      601 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      661 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      721 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      781 tcttgtacaa agtggtgatg tacctcgagc accaccacca ccaccactga gatccggctg
      841 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      901 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      961 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1021 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1081 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1141 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1201 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1261 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1321 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1381 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg
     1441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2101 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2701 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3361 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3421 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     3481 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     3541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     3661 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     3721 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     3781 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3841 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     3901 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     3961 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     4021 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     4081 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     4141 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     4201 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     4261 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     4321 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     4381 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     4441 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     4501 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     4561 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     4621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4681 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     4741 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     4801 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     4861 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     4921 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     4981 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5041 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5101 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     5161 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     5221 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     5281 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     5341 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     5401 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     5461 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     5521 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     5581 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     5641 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     5701 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     5761 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
     5821 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     5881 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     5941 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     6001 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     6061 cgagatccag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
     6121 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatgaa
     6181 acaccatcac catcaccatc accatgagct cgacgcaccg gaagaagagg atcatgtcct
     6241 ggttctgcgc aaaagcaact tcgcggaagc actggccgca cataaatatc tgctggtgga
     6301 attttatgct ccttggtgcg gtcattgcaa agccctggcc ccggagtacg ccaaagccgc
     6361 aggtaaactg aaagctgaag gtagcgaaat ccgcctggca aaggttgatg ctacggaaga
     6421 gagtgacctg gcgcagcagt atggtgtccg cggctatcct acaattaaat tcttccgtaa
     6481 cggtgatacc gcatctccaa aagaatatac cgctggtcgc gaggcggacg atattgttaa
     6541 ctggctgaag aaacgcactg gccctgccgc aaccaccctg cctgatggcg ctgctgccga
     6601 aagcctggtc gaaagtagcg aagttgccgt cattggtttc tttaaggatg tagaatctga
     6661 cagtgccaaa cagtttctgc aagcggcaga ggctatcgat gacatcccgt tcggcatcac
     6721 ctctaacagt gacgtattca gtaaatacca actggataaa gacggcgttg tgctgttcaa
     6781 gaaatttgat gaaggtcgca acaactttga gggtgaggtg accaaggaga acctgctgga
     6841 ttttattaag cacaaccaac tgccgctggt tattgaattt acagaacaaa cggcgccgaa
     6901 aattttcggc ggtgagatta aaacacatat cctgctgttt ctgccgaaga gcgtttctga
     6961 ttacgatggt aaactgagta attttaaaac cgccgcagaa tctttcaaag gtaagattct
     7021 gttcattttc attgatagcg accacacgga caatcagcgt atcctggagt tctttggtct
     7081 gaagaaagag gaatgcccgg ctgtgcgtct gattacgctg gaagaggaaa tgacaaagta
     7141 caagccggag agcgaggaac tgactgcaga acgtatcacc gaattttgtc atcgtttcct
     7201 ggaggggaag attaagccgc atctgatgag ccaggaactg ccggaggatt gggacaaaca
     7261 gccagtgaaa gttctggtgg ggaagaattt tgaagatgtg gccttcgatg agaagaagaa
     7321 tgtgtttgtg gagttctacg ccccgtggtg tgggcactgt aaacagctgg cgccgatctg
     7381 ggacaaactg ggcgaaacgt ataaagatca cgaaaatatt gtgatcgcga aaatggattc
     7441 taccgcgaat gaagtagaag cggtaaaagt acactctttt ccgacgctga aattctttcc
     7501 agcgagcgcg gatcgtactg tcattgatta taatggcgaa cgtactctgg acggctttaa
     7561 gaaatttctg gaaagcggcg gccaggatgg cgcgggcgat gatgatgacc tggaagacct
     7621 ggaagaagcg gaggaaccag acatggagga ggatgacgac cagaaagcgg tcggtaccgg
     7681 atcttacatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     7741 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     7801 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     7861 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     7921 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     7981 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     8041 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     8101 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     8161 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     8221 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     8281 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     8341 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     8401 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     8461 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     8521 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     8581 taaacgcgtg gatccggct
//
