LOCUS       sequence-97127-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JUY
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(614..634)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(647..664)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             674..696
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    711..735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttcgtca tcagtcttcc aacagctgct ccagtgcctg ctgtcttgga tgagtattct
      121 gcaatagcag gctgagctct gtccgaagca gcagtacttc cagtgtgact gtagcaacct
      181 ggtgctcatc tggctcctcc tgcccttcgg ggaagctcaa cccatctggt tgctcctctg
      241 tgtagccata taatcgcagc acatctcggc ccccctgcac agcatccacc gtgctgcgaa
      301 agacagggtt attaaacttg acaccacgcc agtaccgagg ccgctgaggg ctgagaaggt
      361 tgcggccgta tttctccagg atgttcagag ccgtggacag ggtgttgagg tagtttcggg
      421 gctccccatg agcgttgcag cggacaaggc gtgcggcgtc cagctgcagg tagcgggcgg
      481 ctagcggcag agagctggct agtagcggcc ggagctgctc cagggaaaac gcctgcccgg
      541 aatccctcct cagggcgctc gccagctcct cgcgggccac caggaaggcc cgctcctctt
      601 cctcccccgg catgccctgg aaatacaagt tttctctgcc gctgctgtga tgatgatgat
      661 gatgcatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      721 tccgctcaca attcccctat agtgagtcgt a
//
