LOCUS       sequence-97128-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JWQ
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             656..673
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(726..744)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac ngcagcggca
       61 gagaaaactt gtatttccag ggcttagatg tcaaaatgga tatacatgta ttggaaaatt
      121 ttaagaatta tgctttatta ttaaaacttc aaaaattagc tatgacaata atagcacaac
      181 aaagtaatga ctacgatttg caacaactaa agacagtatt tttatattta gatgaagatg
      241 gaaaaggaaa tataacaaaa aatcaattga aaaaaggttt ggaaaatagt ggattgaagc
      301 ttcctcaaaa ttttgatgta ttacttgatc aaatagatag tgatggtagt ggtagaatag
      361 attatactga atttttagcg gcagcattag acagaaagca tttatcaaag aaactgatat
      421 attgcgcctt tagggtattt gatgtagaca atgatgggga aattacaaca gcagaattag
      481 cacatatact ttataatggc aacaagaaag gaagcataac tcaaaaagat gttaatcaag
      541 ttaaaaaaat gattcaagaa gttgacaaga acaatgatgg aaagatcgac ttttatgagt
      601 tttgcgaaat gatgaaacta aagtattgat gacgaagctt gcggccgcac tcgagcacca
      661 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      721 tgccaccgct gagcaataac tagcat
//
