LOCUS       sequence-97143-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LD6
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(455..472)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             482..504
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    519..543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(543..562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(544..562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(646..665)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttcgtca tcacgtttca aactgggtta agagctccct gatttccggg gccacgtgtc
      121 ttgcggcatt tgcagcctcg gttatagctt tccgatacat ccccttcttc ttacgattaa
      181 atctcagttt gaaaaattca gagaaagggg agagttttga aatagacaag aacgacgtag
      241 tcggagaacc aaaccacgag accttcgctt ctcgccaaga cggctctccg tcctccttct
      301 ggccgagact gatgtcaaga acacgcgccg gccaccaagg aaaaccatgg atcttacccc
      361 agacgatgtc ccccacggcc acagtcctgc cgtcctccgt gatgcactcc gacacgctct
      421 ggccctggaa atacaagttt tctctgccgc tgctgtgatg atgatgatga tgcatggtat
      481 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
      541 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      601 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      661 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      721 gtatggtggc agg
//
