LOCUS       sequence-97202-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2PD0
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             732..749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgttt aactttaaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt aaactattgg gagttaatag ttttgctcta
      121 agacagtttg tggagggata cagaggaagt tatatcccaa gaatgagtcc ttatgaattc
      181 ttgaggaacg taaataatta cattatcgag aataatccta cattagtcga tgggtatgca
      241 gatttctgca aacatatttt tattccaaat tttacagaag caaagcaatc aattgtaaag
      301 atcacaaatg aaaacgaaaa atatattaag acagggtata tttcgaggag agacgaagag
      361 attccagtat tgtcaaggtg gttcccaaag gattccccac ccgcatcaca actaattaaa
      421 agcaagtatt tagatatcat tctttatagt aaagaacagt gtgagaaaga atcaagcatt
      481 atgaactgtt gtctacaaga tattctagat gatagggaaa agaacccgga ctggtatata
      541 atatcaatta aggcgcaaaa tgaaagcttt gaagtcccaa tggagccaat aacaattctt
      601 agaaatactt taatagaaga gggaggtagt ggggtcccat tgaaaagaga aaaatactta
      661 gagtcggtag agttttggaa ggaacatgcc attgtatcta gctgatgacg aagcttgcgg
      721 ccgcactcga gcaccaccac caccaccact gagatccggc tgct
//
