LOCUS       sequence-97274-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PT3 GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_9
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(88..106)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(538..557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttgctcatc tttacctcct ctatcgcgga aattgacagg atcctctccc tatagtgagg
       61 gttattactc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      121 tcatgataat aatggtttct tagacgtcgg aattgccagc tggggcgccc tctggtaagg
      181 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
      241 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcgataaa tgcttcaata
      301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      421 tgaagatcag ttgggtgcac gagtgggtta tatcgaactg gatctcaaca gcggtaagat
      481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      661 catgacagta agagaattat gcagtgctgc cataaccatg a
//
