LOCUS       sequence-97275-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with CD4 ectodomain, no linker domain, G cleavage 
            sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: CD4_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatcatgt gccgagccat ctctcttagg
      121 cgcttgctgc tgctgctgct gcagctgtca caactcctag ctgtcactca agggaagacg
      181 ctggtgctgg ggaaggaagg ggaatcagca gaactgccct gcgagagttc ccagaagaag
      241 atcacagtct tcacctggaa gttctctgac cagaggaaga ttctggggca gcatggcaaa
      301 ggtgtattaa ttagaggagg ttcgccttcg cagtttgatc gttttgattc caaaaaaggg
      361 gcatgggaga aaggatcgtt tcctctcatc atcaataaac ttaagatgga agactctcag
      421 acttatatct gtgagctgga gaacaggaaa gaggaggtgg agttgtgggt gttcaaagtg
      481 accttcagtc cgggtaccag cctgttgcaa gggcagagcc tgaccctgac cttggatagc
      541 aactctaagg tctctaaccc cttgacagag tgcaaacaca aaaagggtaa agttgtcagt
      601 ggttccaaag ttctctccat gtccaaccta agggttcagg acagcgactt ctggaactgc
      661 accgtgaccc tggaccagaa aaagaactgg ttcggcatga cactctcagt gctgggtttt
      721 cagagcacag ctatcacggc ctataagagt gagggagagt cagcggagtt ctccttccca
      781 ctcaactttg caga
//
