LOCUS       sequence-97278-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with CD4 ectodomain, no linker domain, G cleavage 
            sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: CD4_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             192..425
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(501..522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta
       61 cgaaaaacaa ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc
      121 ccgaagaggc ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca
      181 gactgtcgac ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg
      241 aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg
      301 acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg
      361 atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg
      421 gtggaggtac cggcggaggc tccggtggtg gctctatggt gagcaagggc gaggagctgt
      481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      541 gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
      601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg
      661 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      721 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga
      781 cccgcgccga ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg
      841 tcgacttcaa ggaggacggc aacatcct
//
