LOCUS       sequence-97300-e         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human UBE2E3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-UBE2E3i2
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     142..162
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc
       61 tttttaatac tagctacatt ttacatggat aggcttggat ttctataact tcgtatagca
      121 tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt aaagtaattg
      181 tgtgttttga gactataaat atcccttgga gaaaagcctt gtttgctgtg tatatgttat
      241 actgattttc tagagaaatc agtataacat atacacagct tttttctcga gtactaggat
      301 ccattaggcg gccgcgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
      361 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      421 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      481 tgttcccata gtaacgccaa tagggacttt ccattgacgt c
//
