LOCUS       sequence-97309-e         289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human DRAM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-DRAM1i1
FEATURES             Location/Qualifiers
     source          1..289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(100..133)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     137..157
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
       61 taatactagc tacattttac atgataggct tggatttcta taacttcgta tagcatacat
      121 tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
      181 tttgagacta taaatatccc ttggagaaaa gccttgtttg ctgtggtgga atgaatccat
      241 acttctagag agtatggatt cattccacca cagctttttt ctcgagtac
//
