LOCUS       sequence-97340-e          20 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant 
            human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human T (BRACHYURY) sgRNA
FEATURES             Location/Qualifiers
     source          1..20
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacgcgcagt tcgcgctctg
//
