LOCUS       sequence-97343-e         335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human PTPRK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-PTPRKi2
FEATURES             Location/Qualifiers
     source          1..335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(100..133)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     137..157
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
       61 taatactagc tacattttac atgataggct tggatttcta taacttcgta tagcatacat
      121 tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
      181 tttgagacta taaatatccc ttggagaaaa gccttgtttg ccgtattact ttgctgcaga
      241 acttctagag agttctgcag caaagtaata cggctttttt ctcgagtact aggatccatt
      301 aggcggccgc gtggataacc gtattaccgc catgc
//
