LOCUS       sequence-97344-e         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human PTPN11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-PTPN11i1
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..140)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     144..164
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
       61 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa cttcgtatag
      121 catacattat acgaagttat aaacagcaca aaaggaaact caccctaact gtaaagtaat
      181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttggac agatcttgtg
      241 gaacattatt tctagagaat aatgttccac aagatctgtc cttttttctc gagtactagg
      301 atccattagg cggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
      361 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      421 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cat
//
