LOCUS       sequence-97396-e         563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An LV packaging vector expressing eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 563)
  TITLE     Lentiviral vectors for stable ectopic expression or knockdown of WNT
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOTTC155
FEATURES             Location/Qualifiers
     source          1..563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(77..118)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    171..195
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(265..286)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attgtcgatc
       61 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt
      121 cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt
      181 acaagaaagc tgggtctaga tatctcgagt gcggccgctt tacttgtaca gctcgtccat
      241 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      301 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      361 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      421 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      481 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      541 ctccttgaag tcgatgccct tca
//
