LOCUS       sequence-97455-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP with robust performance in protein fusions and useful as FRET 
            donor for mRuby2 in a FRET pair that offers bright fluorescence, 
            dynamic range, and photostability while limiting emissions overlap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3-Clover
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(16..726,727..729,730..732))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(58..79)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     666..687
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(797..816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(798..816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atagggccct ctagacttgt acagctcgtc catgccatgt gtaatcccgg cggcggtcac
       61 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      121 atggctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      181 ctggtagtgg tcggcgagct gcacgctgcc gtcctcaacg ttgtggcgga tcttgaagtt
      241 agccttgatg ccgttcttct gcttgtcggc cgtgatatag acgttgtggc tgttgaagtt
      301 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      361 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtaggtacc
      421 gtcgtccttg aaagagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      481 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag caggccacgc cgtagccgaa
      541 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      601 ggtcagcttg ccgttggtgg catcgccctc gccctcgccg cggacgctga acttgtggcc
      661 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      721 cttgctcacc atgccgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg
      781 taccaagctt gggtctccct atagtgagtc gtattaattt cgataagcc
//
