LOCUS       sequence-97475-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV zinc finger donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAVS1 SA-2A-puro-pA donor
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(354..656)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(456..475)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     779..801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     793..810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     794..810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagagc ttgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
      181 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
      241 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
      301 tacgtgaaaa ggatctaggt gaagatcctt tttgataatc tcatgcctga catttatatt
      361 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
      421 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
      481 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
      541 cgtgcactgg ccagggggat caccatccgt cgccccggcg tgtcaataat atcactctgt
      601 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgccgt
      661 acgtataggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      721 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      781 cagtcacgac gttgtaaaac gacggccagt gaa
//
