LOCUS       sequence-97558-e         418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA800.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 418)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-splitTVA800
FEATURES             Location/Qualifiers
     source          1..418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..36
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    87..120
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 atataacttc gtataatgta tgctatacga agttatgcag aatggtagct ggattgtagc
       61 tgctattagc aatatgaaac ctcttaataa cttcgtataa agtatcctat acgaagttat
      121 ggcgcgccct atcagtccca tctcaccagc tcacagcaaa agatccctgc agtgatcagc
      181 atccacatgc ggccgtgatt cctggctggc agagcacgtc caggagcagg gacagtggga
      241 gcctctgtgc cgttgtccgt gggcaccgcg ggggtcgcgc tggtcccgca gccccactcg
      301 tcccgcccgt cgtcgcagtc ggggtgtccg tcgcacagcc agtcctgcgg gtaacactcc
      361 ccgtgggcac cgggcggctc cgagcagcgg aactgaccgg gggggcaacg ggacaaag
//
