LOCUS       sequence-97623-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK1_eGFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(51..764)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(51..764)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(91..111)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     706..734
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             772..794
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tttgtatagt
       61 tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct
      121 ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt
      181 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
      241 cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg
      301 tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg
      361 tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct
      421 tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt
      481 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt catatgatct
      541 gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt tggccatgga
      601 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
      661 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
      721 agaattggga caactccagt gaaaagttct tctcctttac gcatatgtat atctccttct
      781 taaagttaaa caaaattatt tctagaaagt taaacaaaat tatttgtaga
//
