LOCUS       sequence-97673-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Puro DEST (694-6)
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(137..142,143..160,161..167)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(287..306)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
       61 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      181 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      241 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      301 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      361 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      421 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      481 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      541 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      601 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
      661 caatccctgg gtgagtttca cca
//
