LOCUS       sequence-97773-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eef1a (EF1alpha) promoter driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Eef1a-1955/-1>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgcggataa gtttgtcgtt ttcgtcgtac ttggtattca tgcgtgagtc aaggatttgg
       61 gcgacatgtt tagtaatctg ccgagtctcc acaagttgcc gtttaatgaa tcctgctttg
      121 tccagttcgc tcagtccgcc cctctcagcc ttagtgagat tgtcgaactt ccgctgagtg
      181 atcagctttg cgttcagaag ctggcgccaa tagttcttca tctttttcac gacttcctct
      241 gagggcacgt tatctgactt tccccggttc ttgtctgagc gagtcagcac cttattgtca
      301 attgagtcgt ctttgaggaa gctctgaggg acgatgtggt ccacgtcgta gtctgagagc
      361 ctgttgatat caagctcttg gtccacatac atatcgcgtc cattttgaag gtagtacaga
      421 tagagcttct cattctgaag ctgggtgttt tccaccgggt gctctttaag gatctgtgac
      481 ccgagctcct taatcccttc ctcaattctt ttcatccgct ccctgctgtt cttctgtccc
      541 ttctgggtag tctggttttc cctggccatc tcgatgacaa tgttctctgg tttatggcga
      601 cccatcacct tgaccagctc gtccacgact ttcacggtct ggagaatacc cttcttaata
      661 gcgggtgaac cagccagatt tgcgatatgc tcgtgcagtg agtcaccttg tccagacact
      721 tgggccttct ggatgt
//
