LOCUS       sequence-97789-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for CRISPR disruption of TGME49_290860..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Behnke MS, Khan A, Sibley LD
  TITLE     Genetic mapping reveals that sinefungin resistance in Toxoplasma 
            gondii is controlled by a putative amino acid transporter locus that
            can be used as a negative selectable marker.
  JOURNAL   Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
  PUBMED    25480939
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell. 2014 Dec 5. pii: EC.00229-14."
COMMENT     Sequence Label: pSAG1::CAS9-U6::sg290860-6
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(39..113)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     695..711
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     695..711
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tcaaaaaagc accgactcgg tgccactttt
       61 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aaccacatcg
      121 ctgtcagacc aataacttga catccccatt taccagaagg caaacacccc cttcggggac
      181 gaggtgaccc tgcgcgacag aaagcccctt cgaagagcgc acagggagga agcaggcctc
      241 tgcaggtcgc catttgaaaa tctgacagaa ctgatggaaa tatgattctt gtcagagaag
      301 acattcgaga gttcgaaggt ttcccccttg gctctacata tcccagtgtc tcgcgttctg
      361 caggaggcgc gtcaggccta ggatgcaata ttggcgccca attcacagtg cagcggcgca
      421 gccgtcgcaa cacttcgcag cgtgaaagac ttatcgctct ccgcctctcg atggttcggg
      481 atgttcgctt cgagtgtatg ctgcgcaagc tttcgcgctt gggtacgtta atttgaaatc
      541 tttcgcgttt cccgtgtgtc ctcagtgtct ccaacatgtg gccgcggtgc aatcgtcccc
      601 acgacgattt caactcgcgc actttgtctc gtcgcaccct cccggaaata cagcgtgctt
      661 ctgcttactt gagctccacc gcggtggcgg ccgctctaga actagtggat ccccctcggg
      721 ggggcaagaa ttgtgttaac cggttcgact aaaacaactg atagctgtct tgtgtattga
      781 cccatgtggc gcgtgtgtcg acggcgatca acatcagcca ggtccgccac acatccgttc
      841 agagattcgg acccagtcat aaactttcgc tgcttgctgc gaaacactcc tatttagagt
      901 cggccggttt ttgtggatgt gacacctgca agcc
//
