LOCUS       sequence-97790-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for CRISPR disruption of TGME49_290860..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Behnke MS, Khan A, Sibley LD
  TITLE     Genetic mapping reveals that sinefungin resistance in Toxoplasma 
            gondii is controlled by a putative amino acid transporter locus that
            can be used as a negative selectable marker.
  JOURNAL   Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
  PUBMED    25480939
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell. 2014 Dec 5. pii: EC.00229-14."
COMMENT     Sequence Label: pSAG1::CAS9-U6::sg290860-6
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..42
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     27..43
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tttacatccg
       61 ttgccttttc cacggtccgt gatttcatgt gcgtgcagct tcaaagactg gtcgttgcga
      121 ctaataagac tgcagtgaca ggtcgaatgg tgggcacctt gctgatgact atctactgca
      181 aagtctgaga caacgaacga aacttcccac acgaggcatt tgaaactgac ggtgtctagg
      241 taatatgcac tgcaagacac ggtactgggg cctcgctgaa ttaggggccg atctcgttgc
      301 cctatcagtg ctcacagtgc cgcaacgtaa caccagggca ggttcttgac agtggcaaca
      361 atgtgcgacg ggcgtgtgaa cgtttcgtag tcatagcgct agcacgtacc tagccacatg
      421 gtcgtgagga gctttaccat gcgtctagaa ggtggatgcg ggacacgcct tcctggcctt
      481 tggctcccga gacgcgtgtt ctaaccacaa accttgagac gcgtgttcca accacgcacc
      541 ctgacacgcg tgttccaacc acgcaccctg agacgcgtgt tctaaccacg caccctgaga
      601 cgcgtgttct aaccacgcac cctgagacgc gtgttctgcc gcacaatgtg cacctgtagg
      661 aagctgtagt cactgctgat tctcactgtt ctcggcaagg gccgacgacc ggagtacagt
      721 ttttgtgggc agagccgttg tgcagctttc cgttcttctc ggttgtgtca catgtgtcat
      781 tgtcgtgtaa acacacggtt gtatgtcggt ttcgctgcac cacttcatta tttcttctgg
      841 ttttttgacg agtatgcatc tagacaaaat ggacaagaag tacagcatcg gcctggacat
      901 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
      961 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggcgccct
     1021 gctgttcgac agcggagaaa cagccgaggc c
//
