LOCUS       sequence-97843-e         356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  silence doxycyclin inducible pUltra-dox (also expresses luciferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 356)
  TITLE     pUltra plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf-luc-RTTA3
FEATURES             Location/Qualifiers
     source          1..356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..101
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 taaaggccaa gaagggcgga aagatcgccg tgagatccgg ctccggcgag ggcaggggaa
       61 gtctactaac atgcggggac gtggaggaaa atcccggccc agctagtatg tccagactgg
      121 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
      181 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
      241 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
      301 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgcc
//
