LOCUS       sequence-97923-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts GFP immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-GFP
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..66)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gctcaccatt ggcccgggat tctcttcgac atcccctgct tgtttcaaca gggagaagtt
       61 agtggcgcta gctatttcac ctggtggagt cacagagtag ttcaggaata attccaaggc
      121 ttgtggggtg ctaaaatgcg catggctttc cctagtggct tccaaattca cctccaaatc
      181 actggcagag aagttttgct gcaactgtac gtaaggctgc agaaagtcct ccccgaagtt
      241 atggagcgga gcagcattcc aaggctggcc gttccaggac tgagcggtcc aggcctggct
      301 gctccaagtt gggttggtcc aggtctggtt gttccaagtt gggttggtcc aggtctggct
      361 gctccaagtt gggttggtcc aagtctggct gccccacatg gaaaggcttc cagatgcgtt
      421 caccagatag ccctggggat agctgcaatg gatgctggga tactccactg gtgctgagcc
      481 cttctgaaag aggtgattaa tgcaatgagt gaaaagggga ctctaaagat gtaaactgca
      541 gaaatactga aaagattagc atggccgcac aggccgttaa ccccagcact cctgagacta
      601 ggcaggcaga tctctgactg ccagggctac atagagagag cctgcctcaa agaaccaaag
      661 atcacaagaa agagtgcgtg gaggaagagg gatgggaaag ggacaggact aatggacata
      721 gatggatcag gatgacactg ggaagcaaag gcagagccac tgactgctc
//
