LOCUS       sequence-97946-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable expresses luciferase in mammalian cells by retrovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  TITLE     PLXIN-Luciferase, stable expresses luciferase in mammalian cells by 
            retrovirus system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLXIN-Luc
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..219
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(299..716)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(315..331)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(347..369)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tgcacaaacc acacacggca taagattgag agagttttca ctgcatacga cgattctgtg
       61 atttgtattc agcccatatc gtttcatagc ttctgccaac cgaacggaca tttcgaagta
      121 ctcagcgtaa gtgatgtcca cctcgatatg tgcatctgta aaagcaattg ttccaggaac
      181 cagggcgtat ctcttcatag ccttatgcag ttgctctcca gcggttccat cttccagcgg
      241 atagaatggc gccgggcctt tctttatgtt tttggcgtct tccatggatc cgaattccgg
      301 cgcctagaga aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag
      361 gttcaagggg gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa
      421 agggcttgac ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga
      481 cctggtctgg gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga
      541 ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag
      601 agcagaaggt aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct
      661 cgacatcttt ccagtgacct aaggtcaaac tttaagggag tggtaacagt ctggccctaa
      721 ttttcagaca aatacagaaa acacagtcag acagagacac acagaaccga tgctgcagcg
      781 ctgcaagcag accagacgcg cggctttcgg ttcccaaatc ggaaagcaaa atttcagacg
      841 gaggcgggaa ctgttttagg ttcctcgtct cctaccagaa ccatcataat cctgaccggg
      901 gtcggaatcc cacattcgaa ctcccctttc cttcagggcc ggggccacaa aaagacgcg
//
