LOCUS       sequence-98022-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a genomic fragment that is not active in human
            pancreatic islets, a minimal promoter and a luc2 gene. Can be used 
            as negative control in reporter assays performed in human pancreatic
            islets..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23 C5_12
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcccgtcga cgaattctgc agatatcaca
       61 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccagcccttg gtcaagaata
      121 gttataatta tcagttcaaa ttatataaaa attattgctc ctggaattct ttttcatttt
      181 ttttgttatt gctgtttagt aaagtctatt tcttcataaa tactaagaaa atccaggcct
      241 tggtcagata gtggaatttt agtggaattc agattagtgc agtttaatca taagaaccat
      301 ttacattttc ccatttgtgt taagttctag ctatttgccc tgcatataga gaatatagag
      361 tagatagctt tcaatatcat cttatgtgta atttagtgac tttgagacta gatgtgcctg
      421 tctggaacaa ctgtctcatt aatcattagg attcttggat tattttcttg acctggtata
      481 gtcattgtca gaaaaatttt ggcagttgtt tagtgccatc tagtgggcac tttcttgtga
      541 aaaacaaaaa actcttattt aacagaaagg attgttgcat tttattattt ataaaatcat
      601 ggtatgcaat attaatttca gattttataa attaggatat aatagagcta tcaaatg
//
