LOCUS       sequence-98033-e         395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted SNAP(f)-tag and histone-GFP, 
            conditional.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Kohl J, Ng J, Cachero S, Ciabatti E, Dolan MJ, Sutcliffe B, Tozer A,
            Ruehle S, Krueger D, Frechter S, Branco T, Tripodi M, Jefferis GS
  TITLE     Ultrafast tissue staining with chemical tags.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 25. pii: 201411087.
  PUBMED    25157152
REFERENCE   2  (bases 1 to 395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 25. pii: 201411087."
COMMENT     Sequence Label: pAAV-myrSNAP-CON
FEATURES             Location/Qualifiers
     source          1..395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(174..195)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attgggattc gaacatcgat tgaattcata acttcgtata gcatacatta tacgaacggt
       61 atcactatta cttactatca cctattatca cggccgcccg gatccgatga gcttgatgat
      121 ctgagtccgg acttgtacag ctcgtccatg ccgtgagtga tcccggcggc ggtcacgaac
      181 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      241 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      301 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc
      361 ttgatgccgt tcttctgctt gtcggccatg atata
//
