LOCUS       sequence-98038-e        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of NLS-mCherry (control plasmid). Confers 
            resistance to Puromycin. Expression is inducible in T-REx cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B,
            Frit P, Calsou P
  TITLE     DNA damage triggers SAF-A and RNA biogenesis factors exclusion from 
            chromatin coupled to R-loops removal.
  JOURNAL   Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 
            10.1093/nar/gku601. Epub 2014 Jul 16.
  PUBMED    25030905
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku601"; journalName: "Nucleic Acids Res"; date: 
            "2014-10-1- 1"; volume: "42"; issue: "14"; pages: "9047-62"
COMMENT     Sequence Label: pICE-NLS-mCherry
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     62..86
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             178..198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             214..924
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(359..377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     627..646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(992..1009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 taagcagagc tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac
       61 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      121 atagaagaca ccgggaccga tccagcctcc ggcaagcttg ccaccatgac gcgtggcccc
      181 aagaaaaagc ggaaagtggg accggtcgcc accatggtga gcaagggcga ggaggataac
      241 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      301 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      361 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      421 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      481 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      541 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      601 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      661 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      721 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      781 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      841 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      901 ggcatggacg agctgtacaa gtaaagcggc cgcgaattcg gatccggtac cctcgagacg
      961 cgtctgcagt ctagataaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     1021 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aagg
//
