LOCUS       sequence-98103-e        1311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule fused to cohesin type III domain
            to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced ybbR tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-CohIII-CBM-HIS-ybbR
FEATURES             Location/Qualifiers
     source          1..1311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1228..1245
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1252..1275
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgggtaccg cgctcacaga cagaggaatg acttacgatc tcgatcctaa ggatggatca
       61 tctgctgcta caaagccagt tctcgaagta actaagaagg ttttcgacac agctgctgat
      121 gctgctggcc agactgttac agttgaattc aaggttagcg gtgcagaagg caagtacgct
      181 acaactggtt atcacatcta ctgggatgag agacttgaag ttgttgcaac caagacaggt
      241 gcatacgcta agaagggcgc agctcttgaa gattcaagct tagctaaggc tgagaacaac
      301 ggtaacggtg ttttcgttgc ttcaggtgct gatgatgatt ttggtgctga cggtgtaatg
      361 tggactgttg aactcaaggt tccagcagac gctaaggctg gcgatgtata tccaatcgac
      421 gttgcttatc agtgggatcc ttcaaagggt gaccttttca cagataacaa ggactcagca
      481 cagggcaagc tgatgcaggc ttacttcttc acacagggta tcaagtcatc ttctaatcct
      541 tcaacagatg agtacctcgt aaaggctaac gctacatatg ctgatggtta cattgcaatc
      601 aaggctggtg agccaggcag tgtagtacca tcaacacagc ctgtaacaac accacctgca
      661 acaacaaaac cacctgcaac aacaataccg ccgtcagatg atccgaatgc catggccaat
      721 acaccggtat caggcaattt gaaggttgaa ttctacaaca gcaatccttc agatactact
      781 aactcaatca atcctcagtt caaggttact aataccggaa gcagtgcaat tgatttgtcc
      841 aaactcacat tgagatatta ttatacagta gacggtcaga aagatcagac cttctggagt
      901 gaccatgctg caataatcgg cagtaacggc agctacaacg gaattacttc aaatgtaaaa
      961 ggaacatttg taaaaatgag ttcctcaaca aataacgcag acacctacct tgaaataagc
     1021 tttacaggcg gaactcttga accgggtgca catgttcaga tacaaggtag atttgcaaag
     1081 aatgactgga gtaactatac acagtcaaat gactactcat tcaagtctgc ttcacagttt
     1141 gttgaatggg atcaggtaac agcatacttg aacggtgttc ttgtatgggg taaagaaccc
     1201 ggtgagctca agcttcctag gtctaggcac caccaccacc accacggatc cctggaagtt
     1261 ctgttccagg gtccggactc tctggaattc atcgcttcta aactggctta a
//
