LOCUS       sequence-98114-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. For expression of transgenes
            under a CMV promoter when a GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack-CMV
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    168..289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(205..224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     259..278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        375..678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acgcggccgc tcgagcctaa gcttctagat aagatatccg atccaccgga tctagataac
       61 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      121 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcggaggtt
      301 tatcgacgat ctgctagtga ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      361 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      421 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      481 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      541 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      601 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      661 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      721 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      781 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      841 g
//
