LOCUS       sequence-98117-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterially expressed GST-tagged human polo-like kinase 1 PBD 
            AA365-653 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Yeh TY, Kowalska AK, Scipioni BR, Cheong FK, Zheng M, Derewenda U, 
            Derewenda ZS, Schroer TA
  TITLE     Dynactin helps target Polo-like kinase 1 to kinetochores via its 
            left-handed beta-helical p27 subunit.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1023-35. doi: 10.1038/emboj.2013.30. Epub 
            2013 Mar 1.
  PUBMED    23455152
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.30"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1023-35"
COMMENT     Sequence Label: GST-PBD
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..20
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atctggttcc gcgtggatcc ccggaattcg gccggccatc gattgctccc agcagcctgg
       61 accccagcaa ccggaagccc ctcacagtcc tcaataaagg cttggagaac cccctgcctg
      121 agcgtccccg ggaaaaagaa gaaccagtgg ttcgagagac aggtgaggtg gtcgactgcc
      181 acctcagtga catgctgcag cagctgcaca gtgtcaatgc ctccaagccc tcggagcgtg
      241 ggctggtcag gcaagaggag gctgaggatc ctgcctgcat ccccatcttc tgggtcagca
      301 agtgggtgga ctattcggac aagtacggcc ttgggtatca gctctgtgat aacagcgtgg
      361 gggtgctctt caatgactca acacgcctca tcctctacaa tgatggtgac agcctgcagt
      421 acatagagcg tgacggcact gagtcctacc tcaccgtgag ttcccatccc aactccttga
      481 tgaagaagat caccctcctt aaatatttcc gcaattacat gagcgagcac ttgctgaagg
      541 caggtgccaa catcacgccg cgcgaaggtg atgagctcgc ccggctgccc tacctacgga
      601 cctggttccg cacccgcagc gccatcatcc tgcacctcag caacggcagc gtgcagatca
      661 acttcttcca ggatcacacc aagctcatct tgtgcccact gatggcagcc gtgacctaca
      721 tcgacgagaa gcgggacttc cgcacatacc gcctgagtct cctggaggag tacggctgct
      781 gcaaggagct ggccagccgg ctccgctacg cccgcactat ggtggacaag ctgctgagct
      841 cacgctcggc cagcaaccgt ctcaaggcct cctaaggcgc gccagtcgac tcgagcggcc
      901 gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatga
//
