LOCUS       sequence-98297-e       12697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enocdes Camsap1L1 fused to the SunTag for single molecule imaging .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro
FEATURES             Location/Qualifiers
     source          1..12697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     720..740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        725..769
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    771..789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    792..810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     820..844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      954..963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             4003..4011
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5460..5525
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5541..5606
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5622..5687
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5703..5768
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5784..5849
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5865..5930
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5946..6011
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6027..6092
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6108..6173
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6189..6254
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6270..6335
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6351..6416
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     6432..6454
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     6455..6477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             6480..6545
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6561..6626
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6642..6707
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6723..6788
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6804..6869
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6885..6950
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             6966..7031
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             7047..7112
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             7128..7193
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             7209..7274
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             7290..7355
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             7371..7436
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     misc_feature    7507..8059
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7651..7668)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7878..7897
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8071..8670
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8071..8090)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8567..8587
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8708..8725)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8714..8938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8984..9412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9071..9090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9281..9302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9421..9441)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9426..9755
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9606..9741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9668..9687
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9803..9850
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9869..10243
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10373..10494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10410..10429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10464..10483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10543..10559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10543..10559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10556..10578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10567..10583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10591..10597,10598..10615,10616..10621))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10636..10657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10774..10791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10945..11530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11025..11044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11701..12492,12493..12561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12324..12343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12562..12666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcag ctcagctttc tagaccgcgc
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
      781 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgg actctagcgt ttaaacttaa gcttggtacc gagctcggat ccggccacca
      961 tgggggatgc tgcagacccc agggagatga gaaagacgtt cattgttcca gccatcaagc
     1021 cttttgacca ctatgatttc tccagggcca aaatcgcctg caatctggcc tggctggtgg
     1081 ccaaagcctt tgggacagaa aatgtgccag aggaacttca agaaccattt tacacagatc
     1141 agtatgacca ggaacacatc aaaccacctg ttgttaattt gcttctatcg gctgaactat
     1201 actgtcgtgc tgggagtctc attctcaaga gtgatgctgc aaaacccctt ttgggccatg
     1261 atgctgtaat ccaggcttta gcacagaaag gtctttatgt cactgaccag gaaaaattgg
     1321 taactgaacg agatctccac aagaaaccca tacagatgag tgcacatttg gccatgatcg
     1381 ataccctcat gatggcttat actgtagaaa tggtcagtat agaaaaagta attgcgtgtg
     1441 ctcagcagta ttcagctttt tttcaagcca cagatctgcc ctatgatatt gaggacgctg
     1501 tcatgtactg gataaataag gtaaatgaac atttgaaaga cataatggaa caagaacaaa
     1561 aactgaaaga acatcacaca gttgaagctc caggaggtca aaagtctcct tccaaatggt
     1621 tttggaaact ggttccagct cgttatcgga aagagcaaac attgcttaag caactgcctt
     1681 gcattccatt ggtagaaaat ttgttgaagg atgggacaga tggctgtgca ttagctgccc
     1741 ttattcattt ttactgtcct gatgttgtca gattagagga tatttgtttg aaagaaacta
     1801 tgtctttggc tgatagcctg tataatctgc agctgattca agaattttgc caagaatact
     1861 tgaaccagtg ttgccatttc actctggaag atatgctcta tgctgcttca tccataaaga
     1921 gtaattattt ggtgttcatg gcggaactgt tctggtggtt tgaagtggtg aagccgtctt
     1981 ttgtacagcc tcgtgttgtt cgtccacaag gagctgaacc tgtaaaagat atgccttcaa
     2041 ttcctgtctt gaatgctgcc aaaagaaatg tcttagatag tagttctgac ttcccttcaa
     2101 gtggggaagg agctacattt acacagtctc atcatcattt gccttctagg tattcacgtc
     2161 cccaggctca ttcttcagcc tcaggaggaa ttagaaggtc ttcatctatg tcttatgttg
     2221 atggcttcat agggacatgg cccaaagaga aaagatcatc agtgcatggc gtatcatttg
     2281 atatttcttt tgataaagaa gatagtgtac agagatccac tccaaaccga ggaatcactc
     2341 gttctattag taatgaagga cttactctga acaacagtca tgtatctaaa cacattagga
     2401 aaaatttgtc cttcaagcca ataaatggag aagaggaagc agagagcatt gaagaagaac
     2461 ttaatataga ttctcacagt gacctcaaat cttgtgtgcc ccttaacaca aatgaactaa
     2521 attctaatga gaatattcat tacaagcttc caaatggagc tttacaaaat agaatacttc
     2581 ttgacgagtt tggcaatcag atcgagacac caagcattga agaagcatta caaataattc
     2641 atgatactga aaaatctcct catacacctc agccagacca aattgctaat ggcttctttc
     2701 ttcatagtca agaaatgagt atcttaaatt caaatatcaa gttaaatcaa tctagtcctg
     2761 ataatgtaac tgatacgaaa ggtgccttga gtcccataac tgacaatact gaagtagaca
     2821 ctggaattca cgttccttca gaagatattc ctgaaactat ggacgaagat tcttcgttga
     2881 gagattatac tgtaagcttg gactctgaca tggatgatgc atctaaattt cttcaggatt
     2941 atgatattcg aactggcaac accagggaag ctttgagtcc ttgtccaagt actgtaagta
     3001 ccaagtctca gccaggcagc agtgcttctt ctagttctgg agttaaaatg accagctttg
     3061 ctgaacaaaa attcaggaaa ctgaatcata ccgatggaaa aagtagtgga agcagttctc
     3121 aaaaaactac accagaaggc tctgaactta atattcctca tgtggttgct tgggcacaaa
     3181 ttccagaaga aacagggctt ccacagggac gggacactac ccagctgttg gcctctgaaa
     3241 tggtgcatct taggatgaaa ctagaagaaa agaggcgtgc tatagaagcc cagaaaaaga
     3301 aaatggaagc tgcttttacc aaacagagac agaaaatggg aaggacagca ttccttactg
     3361 tagtgaaaaa gaaaggggat gggatatctc ctctacgaga ggaagcggcg ggtgcagaag
     3421 atgagaaagt atatactgat cgagcaaaag aaaaggaatc acaaaaaact gatggacaaa
     3481 ggagcaagtc actggcagat ataaaagaga gcatggagaa tcctcaagcc aaatggctaa
     3541 agtctccaac tacacctatt gatcctgaga agcagtggaa cctggcaagc ccctcagaag
     3601 aaactttaaa tgaaggagag attttagaat ataccaaatc cattgaaaag ttaaattcat
     3661 ccctgcattt tctacaacaa gaaatgcaac gcttgtcact tcagcaggag atgttaatgc
     3721 agatgagaga gcaacaatct tgggtgattt cacctccaca accctctcca cagaaacaga
     3781 ttcgagattt taagccttct aagcaggcag gcctgtcatc agccattgca ccattctcct
     3841 cagactcccc tcgtcctact cacccatctc cacagtcttc taacaggaaa agtgcatctt
     3901 tttctgttaa aagtcaaagg actcctaggc caaatgagtt aaaaataaca cctttgaatc
     3961 gaaccttgac acctcctcgg tctgtggata gccttcctcg gttaaggagg ttttcaccaa
     4021 gtcaagttcc tattcaaact aggtcatttg tatgttttgg ggatgatgga gaacctcagt
     4081 taaaggaatc caaacctaaa gaggaagtta aaaaggagga attggaatcc aaagggactt
     4141 tggaacagcg tggacataat ccagaagaaa aggaaatcaa accttttgag tcaacagtct
     4201 ctgaagtcct atcactgcct gtcacagaga ctgtatgtct gacaccaaat gaggaccaat
     4261 tgaatcaacc cacagaaccc cctcctaaac ccgttttccc acccactgct ccaaaaaatg
     4321 ttaatctgat tgaagtttcc ctctcagatt tgaaaccccc tgaaaaggct gatgtacctg
     4381 ttgaaaaata tgatggagaa agtgataaag aacaatttga tgatgaccag aaagtatgct
     4441 gtggattctt ttttaaggat gatcaaaaag cagaaaatga tatggcaatg aaacgggcag
     4501 ctttgttgga gaaaagatta agaagggaaa aggaaactca gctccggaaa caacagttgg
     4561 aagcagaaat ggagcataag aaggaggaaa caaggcgtaa aactgaggaa gaacgtcaga
     4621 agaaagaaga tgagagagca cgcagagaat ttattaggca agaatatatg aggcggaaac
     4681 aactgaaact aatggaagat atggatacag taattaaacc ccgtcctcaa gtagtaaaac
     4741 aaaaaaaaca gcgaccaaaa tctattcaca gagatcatat tgaatccccc aaaacaccaa
     4801 taaagggtcc tccagtctct agcctttctt tggcatcgct gaacacgggt gataacgaga
     4861 gtgtacattc aggcaagagg acgccaagat cagagtctgt agaaggcttc ttatctccaa
     4921 gtcgttgtgg cagtcgaaat ggagaaaaag actgggagaa tgcatcaaca acttcttcag
     4981 tggcttctgg aacagaatat acaggaccaa agctctacaa agaacccagt gcaaaatcca
     5041 ataagcacat aatacaaaat gctttagctc attgctgttt ggctggaaaa gtaaatgaag
     5101 gtcagaagaa aaaaatactg gaggaaatgg agaaatcaga tgccaacaac ttcttaatct
     5161 tgttccggga ttcaggatgc cagttcagat ctttatacac ttattgccca gaaactgaag
     5221 aaatcaataa actgactggg ataggcccta aatctatcac taaaaaaatg attgaaggac
     5281 tttacaaata taattctgac aggaaacagt ttagccacat acccgctaaa actttatctg
     5341 ccagtgttga tgcaattacc attcatagcc atttatggca gaccaaaaga ccagtaacac
     5401 ccaaaaaact tttacccact aaggcaggtg tacaaagcgg tccgggaggt tctggaggat
     5461 tgttgcccaa aaactaccac ttggagaacg aggtcgcaag gttgaaaaaa ttggtcggtg
     5521 agcgtggcgg ctcaggcggc cttcttccaa aaaattatca tcttgaaaat gaagttgcta
     5581 gacttaaaaa acttgttgga gaaagaggcg ggagcggcgg cctgctgccc aagaactacc
     5641 acctggagaa cgaggtcgcc cgcctgaaga agctggtcgg cgagcgcggt ggttctggag
     5701 gtttgctccc aaagaactac cacttggaaa acgaagtcgc aagattgaag aagttggtcg
     5761 gtgaaagagg aggaagcgga ggattattac ctaaaaatta tcatttggaa aatgaagttg
     5821 ctagattgaa gaagttggtt ggagagagag gggggtctgg aggactacta ccgaaaaact
     5881 accacctaga gaacgaggtg gcccgactaa aaaagctagt gggcgagcgc ggcggcagtg
     5941 gcggcctcct ccccaagaat taccacctcg aaaatgaagt agcaaggctc aagaagctcg
     6001 taggagaaag aggtggttcc gggggactcc ttccaaaaaa ctatcatctt gaaaacgaag
     6061 ttgcacgtct taaaaaactt gttggcgaac gtggcggctc tggcgggctg ctgcccaaga
     6121 attaccacct ggaaaatgaa gtggcccgcc tgaagaagct ggtgggcgaa cgcggaggca
     6181 gtggaggcct actaccgaag aactaccacc tagagaacga ggtggcacgc ctaaagaagc
     6241 tagtgggcga gcgcggcggc tcgggtggtt tattacctaa gaattatcat ttagaaaatg
     6301 aagtagctcg attaaagaag ttagtaggtg aacgtggtgg gtctggtggt cttcttccga
     6361 agaactacca ccttgagaac gaggtagctc gtcttaagaa acttgtaggt gagcgtggtg
     6421 gaagcggtgg tgggctggca agccacgttt ggtgccggga gctgcatgtg tcagaggaac
     6481 ttttgccaaa gaattatcat cttgagaacg aagtggctcg tcttaagaaa ctcgtaggag
     6541 aaagaggtgg cagtggaggt ctgcttccca agaattacca cctggaaaat gaggtagcta
     6601 gactgaaaaa gctcgtaggc gagcgcgggg gaagtggggg attgctgcca aaaaattatc
     6661 atttggagaa cgaagtagca cgactaaaga aattagttgg ggagaggggg ggatcgggtg
     6721 gcttactccc gaaaaattat catctcgaaa acgaagtggc tcggctaaaa aagttagttg
     6781 gagagcgtgg cggttctgga ggcctattac caaaaaacta ccacctcgaa aatgaggtgg
     6841 cacgcttaaa aaagctggtc ggagaacggg gaggcagtgg tggcctacta cccaagaatt
     6901 atcatcttga gaacgaggta gcgcgtttga agaagctcgt cggtgaaaga ggtggctcag
     6961 gagggctgct cccaaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagttag
     7021 tgggcgagag gggaggcagt ggtgggctac ttcctaagaa ttaccacctc gaaaacgaag
     7081 tagctcgatt aaagaaattg gtcggtgaga gaggtgggtc gggtggctta ctgccgaaaa
     7141 attatcatct ggaaaatgag gtagcgagac taaaaaagct cgtaggtgag agagggggtt
     7201 ctggcggctt gctaccaaaa aattatcatc ttgagaacga agttgctagg ctcaaaaagc
     7261 tggttggaga gcgcggcggc tcaggcggtc tgttacccaa aaactaccac ttggaaaatg
     7321 aggttgccag attgaagaaa ctggtcggcg agcgcggtgg aagtggagga ctacttccta
     7381 aaaattatca tttggagaac gaagtcgcta gactgaaaaa gttggttggg gaacgaggag
     7441 ggtctggcgg caagctagtc gaattcgaca cgtaggctag cttagtaggc ggccgccccc
     7501 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     7561 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     7621 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7681 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7741 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7801 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7861 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7921 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     7981 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     8041 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     8101 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8161 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     8221 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8281 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8341 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8401 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8461 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8521 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8581 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8641 tggtgcatga cccgcaagcc cggtgcctga ctcgagtcta gagggcccgt ttaaacccgc
     8701 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8761 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8821 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8881 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8941 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9001 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9061 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9121 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9181 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9241 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9301 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9361 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9421 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9481 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9601 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9661 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9721 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9781 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9841 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     9901 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     9961 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    10021 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    10081 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    10141 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    10201 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    10261 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10321 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10501 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10621 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10681 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11461 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12661 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
