LOCUS       sequence-98368-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B3-responsive fluorescent reporter plasmid containing a 
            STOP-cassette flanked by B3-recognition sites followed by ZsGreen 
            CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-b3STOPb3-ZsGreen
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    277..411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    531..665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 catgttcatg ccttcttctt tttcctacag atccttaatt aataatacga ctcactatag
       61 ggggtcgacc cgccaccggt tgcttaagaa ttacttattc ttaagcaacc tcgcgatgaa
      121 taaatgaaag cttgcagatc tgcgactcta gaggatctgc gactctagag gatcataatc
      181 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      241 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      301 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      361 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgcgactct
      421 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      481 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      541 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      601 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      661 ggatctgcga ctctagagga tcataatcag ccataccac
//
