LOCUS       sequence-98428-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed murine IFITM3-C72,105A with an N-terminal HA tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, Lopez CB, Hang HC
  TITLE     Palmitoylome profiling reveals S-palmitoylation-dependent antiviral 
            activity of IFITM3.
  JOURNAL   Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 
            2010 Jul 4.
  PUBMED    20601941
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.405"; journalName: "Nat Chem Biol"; date: 
            "2010-08"; volume: "6"; issue: "8"; pages: "610-4"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-C72,105A
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..67
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    545..679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(569..588)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     623..642
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(790..806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(790..806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(803..825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    814..830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(838..844,845..862,863..868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    883..904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctctaaaag ctgcggaatt gtacccgcgg gcccaccatg tacccatacg atgttccaga
       61 ttacgctctt atggccatgg aggcccgaat tcccatgaac cacacttctc aagccttcat
      121 caccgctgcc agtggaggac agcccccaaa ctacgaaaga atcaaggaag aatatgaggt
      181 ggctgagatg ggggcaccgc acggatcggc ttctgtcaga actactgtga tcaacatgcc
      241 cagagaggtg tcggtgcctg accatgtggt ctggtccctg ttcaatacac tcttcatgaa
      301 cttctgcgca ctgggcttca tagcctatgc ctactccgtg aagtctaggg atcggaagat
      361 ggtgggtgat gtgactggag cccaggccta cgcctccact gctaaggcac tgaacatcag
      421 caccttggtc ctcagcatcc tgatggttgt tatcaccatt gttagtgtca tcatcattgt
      481 tcttaacgct caaaaccttc acacttaagt cgaccgagat ctctcgaggt accgcggccg
      541 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      601 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      661 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      721 ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc
      781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      961 ttaatgaatc ggccaacgcg cgg
//
